Journal: Cell stem cell
Article Title: MicroRNAs Induce a Permissive Chromatin Environment That Enables Neuronal Subtype-specific Reprogramming of Adult Human Fibroblasts
doi: 10.1016/j.stem.2017.08.002
Figure Lengend Snippet: (A) Genome wide expression analysis of miNs and starting fibroblasts by RNA-seq at day 30 of reprogramming. Plot shows the relationship between average gene expression (logCPM) and log fold-change of miNs compared to fibroblasts. A selection of pan-neuronal and fibroblast-specific genes are highlighted in black text. Blue = fold change < −2 log2 p < 0.01 (more abundant in fibroblasts), grey = fold change > −2 log2 < 2 log2 p > 0.01, and red = fold change > 2 log2 p < 0.01 (more abundant in miNs). FDR < 0.01.
Article Snippet: Experimental Model and Subject Details Primary Cell Cultures Primary fibroblasts utilized in this study: 1-year-old male (PCS-201-010, ATCC), 22-year-old female (GM02171, NIGMS Human Genetic Cell Repository at the Coriell Institute for Medical Research), 42-year-old female (F09-238, Washington University in St. Louis School of Medicine iPSC core facility), 56-year-old male (AG04148, NIA Aging Cell Repository at the Coriell Institute for Medical Research), and 68-year-old female (ND34769, NINDS Cell Line Repository at the Coriell Institute for Medical Research).
Techniques: Genome Wide, Expressing, RNA Sequencing, Gene Expression, Selection